DETERMINATION AND DETOXIFICATION OF AFLATOXIN IN CEREAL SAMPLES COLLECTED FROM DIFFERENT AREAS OF LAHORE

Objectives: Aflatoxin contamination of cereals and poultry feed causes significant financial loss poses a risk withserious health implications for both humans animals. Present research was conducted to check the occurrence ofaflatoxins B1 in including pulses evaluate potential different techniques inremoving aflatoxin from cereals. Methodology: 112 cereal samples which comprised varieties used as (yellow splitchickpeas, Barley, sorghum, wheat bran, corn seeds oat) were collected various locations Lahore.Quantitative analyses performed through Thin Layer Chromatography High-Performance LiquidChromatography techniques. Detoxification mycotoxin (Aflatoxin) contaminated carried out throughboth chemical physical methods. Results Conclusion: Aflatoxins found 36% (n=40) samples, with concentrations ranging 2.073to 23.03µg/kg-1. Selective 7 belonging seven food types i.e., yellow split chickpea, barley, wheatbran, mixture rendering negative results, subjected HPLC confirm validatethe results TLC. having declared more sensitive technique gave positive three sevensamples that previously AFB1 TLC analysis. The concentration detectedranges between 0.02-0.42µg/kg-1. studies implementation andchemical methods proved efficacy under variable conditions. Study hence suggested acomprehensive regular national level intensive monitoring surveillance plan is required improve qualityand storage Pakistan.